![]() ![]() 2004 2:802–8.Įquils O, Lu D, Gatter M, Witkin SS, Bertolotto C, Arditi M, et al. Chlamydia and apoptosis: life and death decisions of an intracellular pathogen. Interaction of chlamydiae and host cells in vitro. Evidence for long-term cervical persistence of Chlamydia trachomatis by omp1 genotyping. ConclusionĬHSP60- and cHSP10-induced caspase expression, proinflammatory cytokine production and apoptosis of primary cervical epithelial cells might play a role in the pathogenesis of infertility in women with persistent chlamydial infection.ĭean D, Suchland RJ, Stamm WE. ![]() Transcript levels of IL-1β and IL-18 in cells treated with cHSP60 and cHSP10 were found to be significantly ( P < 0.05) higher in stimulated than in control cells. A DNA microarray study showed significant ( P < 0.05) upregulation of interleukin (IL)-1 β-convertase, and caspase-3, -8 and -9 genes in cHSP60- and cHSP10-stimulated than in control cells as confirmed by real-time RT-PCR and western blotting. ResultsĪfter a 4-h incubation in the presence of recombinant cHSP60 or cHSP10, the number of cells exhibiting annexin V binding activity increased 6- and 5-fold, respectively ( P < 0.05). Further, levels of proinflammatory cytokines (IL-18 and IL-1β) were determined by semi-quantitative RT-PCR. Quantitative measurements of apoptosis were made using cytofluorometry, and apoptosis-related genes were analyzed by microarray, real-time PCR and western blotting. Primary cervical epithelial cells were stimulated with cHSP60 and cHSP10 for 4 h. The potential role of chlamydial heat shock proteins (cHSP) 60 and cHSP10 in apoptosis of primary cervical epithelial cells was investigated.
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